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Terms in this set (24)

Describe how tubes w/anticoagulant should be inverted

Inverted gently & completely 5 to 10 times immediately after sample is drawn

What tests can be affected by glycolysis

- Glucose
- Calcitonin
- Phosphorus
- Aldosterone
- A number of enzymes

How soon after collection should cells be separated from plasma or serum?

No more than 2 hours

How can specimens be maintained at 37 C during transport and handling?

- Warmed in a heel warmer
- Incubator

What is the purpose of chilling a specimen?

- Slows metabolic process
- Keeps analytes stable during transport & handling

What minimal documentation should be included with each specimen delivered to the lab?

- Patient's name
- Patient's hospital number & room number
- Specimen type
- Date & time of delivery to the drop-off area
- Name of person depositing the specimen

What are some disadvantages to the pneumatic tube system?

- Unreliability of system
- Speed of delivery
- Potential for specimen damage during transport
- Cost of alternative

What is the purpose of an accession number?

Unique identifying number used for cataloging a sample in the lab

Explain why it is important that a centrifuge carry a balanced load

The rotor of the centrifuge may spin out of center, which can damage centrifuge & cause samples to break

Describe what aliquots are & how they are prepared

- Small portions of a specimen transferred into separate containers for distribution to a variety of lab departments
- All tubes should be labeled before filling & then capped before delivery to appropriate department
- Aliquots are removed w/any one of several types of disposable pipetting systems

Describe the procedure for removing a stopper

- Place a 4-X 4-inch gauze pad over top
- Pull the stopper straight up, twisting if necessary
- Do not rock it from side to side or pop off

How should stat specimens be transported to the laboratory, as opposed to routine specimens?

- Stat Specimen: immediately after being drawn
- Routine Specimen: within 45 min of being drawn

Explain the purpose of maintaining tubes in an upright position during transportation

- To allow complete clot formation
- Prevent sample contamination due to prolonged contact w/stopper
- Reduce formation of aerosol during uncapping

List the reasons specimens should be delivered under a time constraint to the lab

- Will ensure quality of test results
- Glycolysis & other chemical changes increase as time passes

Name the light-sensitive analytes, & describe how to handle these specimens

- Bilirubin
- Vitamin B₁₂
- Carotene
- Folate
- Urine porphyrin
- Specimen must be protected from light
- The tube must be wrapped in aluminum foil or amber-colored tube must be used

Which of these practices is NOT recommended during specimen transport?
a. Placing the sample in a leak-proof bag
b. Carrying the specimen upright
c. Carrying specimen at a 45-degree angle
d. Labeling samples from outside the hospital with a biohazard symbol

C

Cold agglutinins & cryofibrinogen samples should be
a. chilled before colleciton
b. transported on ice to the laboratory
c. warmed before the collection & transported warm
d. transported at room temperature

C

Infant bilirubins are transported
a. on ice
b. in amber-colored microtubes
c. in a heel-warmer packet
d. without special transport measures

B

Once a cap is removed from a blood tube, the pH
a. may decrease
b. may increase
c. will not change
d. becomes alkaline

B

Which of the following can be centrifuged immediately after collection?
a. Serum separator tubes
b. Clot tubes
c. Thrombin tubes
d. Sodium citrate tubes

D

Which of the following specimens would not be rejected?
a. a CBC collected in a lithium heparin tube
b. An EDTA tube used for a chemistry test
c. A sodium level collected in a sodium heparin tube
d. A cold agglutinin sample transported in a heel-warmer packet

D

Complete blood clotting may take _________ at room temperature.
a. 10 to 15 minutes
b. 20 to 30 minutes
c. 30 to 45
d. 1 hour

C

The major risk of stopper removal is
a. glycolysis
b. hemolysis
c. aerosol
d. clotting

C

Which of the following is used to identify a patient specimen in the laboratory?
a. Name of the collector of the specimen
b. Accession number
c. Specimen type
d. Name of person depositing specimen in the laboratory

B

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Which of the following can be centrifuged immediately after collection?

PhlebFinal.

What tubes can be centrifuged immediately?

The ORANGE, LT GREEN and PEARL tubes can be centrifuged immediately. Remember to invert the LT GREEN & PEARL tubes 8-10 times after collecting to make sure the anticoagulant prevents the blood from clotting. Invert ORANGE & GOLD tubes 5 times to mix clot activator with blood.

How soon after collection should cells be separated from plasma or serum quizlet?

How soon after collection should cells be separated from plasma or serum? No more than 2 hours should pass between collection of the sample and separation of cells from plasma or serum.

Which of the following specimens must be completely clotted before centrifugation?

Red top tubes contain no additives. Red top tubes must be allowed to clot completely (30-60 minutes) prior to centrifugation. Centrifuging the specimen yields serum.