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Approach the patient in a friendly, calm manner. Provide for their comfort as much as possible, and gain the patient's cooperation.

Identify the patient correctly.

Order forms filled out correctly, indicating the test(s) ordered.

Verify the patient's condition. Fasting, dietary restrictions, medications, timing, and medical treatment are all of concern and should be noted on the lab requisition.

Check for any allergies to antiseptics, adhesives, or latex by observing for armbands and/or by asking the patient.

Position the patient. The patient should either sit in a chair, lie down or sit up in bed. Hyperextend the patient's arm.

Apply the tourniquet 3-4 inches above the selected puncture site. Do not place too tightly or leave on more than 2 minutes (and no more than a minute to avoid increasing risk for hemoconcentration). Wait 2 minutes before reapplying the tourniquet.

The patient should make a fist without pumping the hand.

Select the venipuncture site.

Prepare the patient's arm using an alcohol prep. Cleanse in a circular fashion, beginning at the site and working outward. Allow to air dry.

Grasp the patient's arm firmly using your thumb to draw the skin taut and anchor the vein. The needle should form a 15 to 30 degree angle with the surface of the arm. Swiftly insert the needle through the skin and into the lumen of the vein. Avoid trauma and excessive probing.

When the last tube to be drawn is filling, remove the tourniquet.

Remove the needle from the patient's arm using a swift backward motion.

Press down on the gauze once the needle is out of the arm, applying adequate pressure to avoid formation of a hematoma.

Dispose of contaminated materials/supplies in designated containers.

Mix and label all appropriate tubes at the patient bedside.

Deliver specimens promptly to the laboratory.

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Terms in this set (19)

site selection

The preferred site for venipuncture is the antecubital fossa of the upper extremities. antecubital fossa is defined as the ditch or impression opposite of the elbow. The vein should be large enough to receive the shaft of the needle, and it should be visible or palpable after tourniquet placement. vein selection is based on the size and condition of the vein
three major veins are located in the antecubital fossa; they are in order of preference:
1. median cubital vein
2. cephalic vein
3. basilic vein

median cubital vein

this vein is most commonly used for venipuncture procedures. it is large and it does not tend to move when the needle is inserted

cephalic vein

this vein can be more difficult to locate. however, it is the easiest vein to palpate in an obese patient

basilic vein

the vein is the least firmly anchored and located near the brachial artery. if the needle is inserted too deep, you risk puncturing the brachial artery or a nerve

laboratory requisition

a requisition is an order form that specifies what tests are being requested. pre-made specimen labels may be available at your place of employment. labels will include an accession number; a unique tracking number different for each sample

antiseptic

prepackaged 70% isopropyl alcohol pads are most commonly used for collection/collections that require more stringent infection control such as blood cultures and arterial punctures, Chloraprep solution is becoming commonly used as the main antiseptic

tourniquet

slows the venous outflow of blood from the arm causing the veins to bulge thereby making them easier to locate. they should be applied 4-6 inches above puncture site. take care to prevent turning or rolling when tying. it should be flat to insure proper restriction. should not be left on the arm for longer than 1 minute prior to beginning a venipuncture to prevent hemoconcentration and infiltration of blood into tissue may occur. additionally, if a patient has frail skin or excessive arm hair, you may tie over clothing. should be single use only. when it is not available, a blood pressure cuff, or sphygomanometer may be used

gloves

must always be worn when collecting blood specimens or performing any venipuncture procedure

vacutainer tubes

Color-coded for specific tests and available in adult and pediatric sizes. the vacuum draw of the evacuated tube will decrease with temperature. tubes with an additive must be inverted the appropriate amount of times in order to ensure proper mixing of blood and additive

vacutainer needles

These are disposable and are used only once for single-tube draw and multi-draw. needle sizes differ both in length and gauge. the gauge of a needle refers to the diameter or thickness of the needle. the lower the number of the gauge the larger the needle, the higher the number of the gauge the smaller/thinner the needle. needles smaller than 23 gauge are not recommended for drawing blood because they can cause hemolysis. the most common needle size used for venipuncture is 21 gauge, 1.25". a gray rubber sleeve covers the backend of the needle that goes into the needle holder. this gray sleeve inhibits blood from flowing out of the vein, up the needle into the hub/needle adapter during venipuncture

needle adapters

also called the tube holders or hubs. one end has a small opening that connects to the needle and the other end has a wide opening to hold the collection tube. hubs should be single used only. hubs should be disposed of immediately following the blood draw, in the sharps container

winged infusion set

another name for this is butterfly needle. used for venipuncture on small veins such as those in the hand. they are also used for venipuncture procedures on elderly or pediatric patients. the most commonly used butterfly is 23 gauge. the angle of entry in the hand vein with the butterfly should be 5 degrees

needle disposal container (sharps contains)

must be a clearly marked and puncture resistant biohazard disposal container. never recap a needle whether it has a safety or not

recognizing patient injury

during a blood draw either in class or in the clinical setting, if the patient states that the blood draw hurts, tingles, is painful, or otherwise the blood draw should be discontinued immediately. they ay complain their arm is falling asleep or they may be shocked by a sharp pain in the area of the venipuncture. this is likely caused by the aggravation of a nerve and the longer the needle is in contact with the nerve itself the more aggravation there will be. the single most common injury that leads to litigation is nerve damage. if the patient complains of any of the issues above immediately discontinue the draw

lancet

a small pricking needle used to pierce the skin to obtain capillary samples

capillary collection tubes

small tubes, or microcontainers, that are used for capillary collection. they have a wide mouth and often times a scooping edge for easy collection. they are color-coded for specific tests and correspond with the color/additive in vacutainer tubes. these tubes have no vacuum

glass slides

used for samples that require microscopic examination, such as a differential blood smear

glass or plastic capillary tubes

small glass or plastic tubes that are used to collect blood from a finger stick. when the blood is collected, it is then spun in the centrifuge to determine the hematocrit- % of red blood cells. also called the "crit"

requisition forms

every blood collection procedure begins with an order or requisition form from a patient's physician, requesting specific tests. patients cannot order their own tests and results of all tests will go directly to the doctor for review. date sample was drawn, what time it was drawn and by whom should all be noted on the requisition. requisition forms may be computer generated or handwritten, and should include:
-patient's name and DOB
-ordering physician information
-tests requested
-special considerations such as fasting, timed tests, postprandial, STAT etc
-diagnosis code (ICD10 code)

if you find that you have multiple orders for a patient for the same or similar blood tests, it is best to clarify with the ordering physicians or lab manager.

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When performing a venipuncture which vein is used the majority of the time?

The veins most commonly used for venipuncture and venous access are the basilic and cephalic veins as well as their branches and tributaries (Figure 48-4). The veins of the dorsal foot and the distal saphenous veins are the most commonly used veins in the lower extremity (Figure 48-5).

Which vein is most commonly used for venipuncture quizlet?

Median cubital vein: A superficial vein, most commonly used for venipuncture, it lies over the cubital fossa and serves as an anastomosis between the cephalic and basilic veins.

Which vein is the first choice for venipuncture?

The antecubital area of the arm is usually the first choice for routine venipuncture. This area contains the three vessels primarily used by the phlebotomist to obtain venous blood specimens: the median cubital, the cephalic and the basilic veins.

What are the 3 most common veins used in phlebotomy?

In this elbow pit, phlebotomists have easy access to the top three vein sites used in phlebotomy: Median cubital vein. Cephalic vein. Basilic vein.