Explain the two methods that are used to prevent blood in the anticoagulant tubes from clotting.

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The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes
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What are the two methods that are used to prevent blood in the anticoagulant tubes from clotting?
What keeps blood from clotting in tubes?
How can you prevent the collection of blood from clotting?
How does EDTA tube prevent clotting?

The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes

Giuseppe Banfi et al. Clin Chem Lab Med. 2007.

Abstract

Anticoagulants are used to prevent clot formation both in vitro and in vivo. In the specific field of in vitro diagnostics, anticoagulants are commonly added to collection tubes either to maintain blood in the fluid state for hematological testing or to obtain suitable plasma for coagulation and clinical chemistry analyses. Unfortunately, no universal anticoagulant that could be used for evaluation of several laboratory parameters in a sample from a single test tube is available so far. Ethylenediamine tetraacetic acid (EDTA) is a polyprotic acid containing four carboxylic acid groups and two amine groups with lone-pair electrons that chelate calcium and several other metal ions. Calcium is necessary for a wide range of enzyme reactions of the coagulation cascade and its removal irreversibly prevents blood clotting within the collection tube. Historically, EDTA has been recommended as the anticoagulant of choice for hematological testing because it allows the best preservation of cellular components and morphology of blood cells. The remarkable expansion in laboratory test volume and complexity over recent decades has amplified the potential spectrum of applications for this anticoagulant, which can be used to stabilize blood for a variety of traditional and innovative tests. Specific data on the behavior of EDTA as an anticoagulant in hematology, including possible pitfalls, are presented. The use of EDTA for measuring cytokines, protein and peptides, and cardiac markers is described, with an outline of the protection of labile molecules provided by this anticoagulant. The use of EDTA in proteomics and in general clinical chemistry is also described in comparison with other anticoagulants and with serum samples. Finally, the possible uses of alternative anticoagulants instead of EDTA and the potential use of a universal anticoagulant are illustrated.

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What are the two methods that are used to prevent blood in the anticoagulant tubes from clotting?

Clotting of blood is prevented by one of the following methods: Chelation or binding of calcium. Precipitation of calcium making it unavailable to the clotting process.

What keeps blood from clotting in tubes?

EDTA is an anticoagulant additive that coats the inside of the tubes to prevent blood clots from forming.

How can you prevent the collection of blood from clotting?

Preventing Blood Clots.

Wear loose-fitting clothes, socks, or stockings..

Raise your legs 6 inches above your heart from time to time..

Wear special stockings (called compression stockings) if your doctor prescribes them..

Do exercises your doctor gives you..

Change your position often, especially during a long trip..

How does EDTA tube prevent clotting?

Ethylenediaminetetraacetic acid (EDTA) is a well-known anticoagulant since early 1950s and it has certain advantages over other anticoagulants [2]. It inhibits clotting by removing or chelating calcium from the blood.