Centrifuges rotate to create a centrifugal force used to separate particles, liquids and other substances that have different densities. More dense parts move away from the centrifuge's axis while less dense parts move toward the axis. Show Particles at the bottom of the sample are the precipitate. The liquid that stays at the top is called the supernate or supernatant liquid. Depending on the procedure, centrifugation may be performed to capture either the precipitate or the supernate. Common Terms:
An important piece of lab equipment, it is crucial to make sure you are using your centrifuge properly and safely.
Centrifuges are sold with or without the rotors and have many options for adaptors to fit the different size centrifuge tubes on the market. Microcentrifuges can hold tubes as small as 0.2mL while larger models hold 500mL bottles. Centrifuges are even available with refrigeration to keep samples cold during processing. By choosing the correct model for your needs and working safely with it, a centrifuge can be a workhorse in your lab for years to come.
The information contained in this article is intended for general information purposes only and is based on information available as of the initial date of publication. No representation is made that the information or references are complete or remain current. This article is not a substitute for review of current applicable government regulations, industry standards, or other standards specific to your business and/or activities and should not be construed as legal advice or opinion. Readers with specific questions should refer to the applicable standards or consult with an attorney. When operating a centrifuge What happens if the centrifuge is not properly balanced?Centrifuges, which operate at high speed, have great potential for injuring users if not operated properly. Unbalanced centrifuge rotors can result in injury or death. Sample container breakage can release aerosols that are harmful if inhaled. The majority of all centrifuge accidents result from user error.
What do you do with a broken centrifuge?If a primary container has broken in a centrifuge without a closed rotor or bucket, immediately suspend use, notify lab staff and PI and request assistance from the Biosafety Officer.
How do you prevent a centrifuge from vibrating too violently?If the balance is off, the centrifuge can experience violent shaking, which can harm the sensitive mechanical parts within. Not only do the weights of the tubes and tube contents need to be balanced, but if the rotor has removable buckets or adaptors, those will also need to be double-checked for balance.
What is the most common error when using a centrifuge?User error is the biggest cause of centrifuge malfunction. Common causes of centrifuge malfunctions include: Failure to place the lid on the rotor. Failure to properly secure the rotor lid.
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