Microscopes are used to know the exact shape, function, and other features of microorganism, which are invisible from naked eyes though vital from biological aspects. The word microscope is taken from a Greek word where ‘mikros‘ means “small”, and ‘skopeo‘ means “to look at”. Show
The use of lenses started in Europe in the 16th century. It is believed that Dutch spectacles makers Zacharius Jansen and his father Hans were the first to invent the compound microscope in the 16th century. Later, Robert Hooke, Anton van Leeuwenhoek, Joseph Jackson Liste, and Ernst Abbe kept on advancing it and invented the Phase Contrast microscope. A few years later Electron Microscope was developed by Ernst Ruska and Max Knoll, with the use of ‘electrons’ in the microscope instead of visible light which helped in increasing the resolution of the lens along with more magnified and cleared image of an organism. Later with the invention of scanning the tunneling microscope, 3-D images viewing started and this was developed by Gerd Binnig and Heinrich Rohrer. This content will provide the important points which distinguish the Light microscope to that of Electron microscope. Content: Light Microscope Vs Electron MicroscopeComparison ChartBasis for ComparisionLight MicroscopeElectron MicroscopeInvented byIt is believed that Dutch spectacles makers Zacharius Jansen and his father Hans were the first to invent the compound microscope in the 16th century.In 1931 physicist Ernst Ruska and German engineer Max Knoll.Source to view the objectVisible light source .Beam of charged particles i.e. electrons.Lense usedGlass lenses.Electromagnetic lenses.Magnification1000X.10,00,000X.Resolving power0.2um.0.5nm.ScreenProjection screen.Fluorescent screen.VoltageNo need of high voltage electricity.High voltage electric current is required (around 50,000 volts and above).Cooling systemThere is no requirement of cooling system.It has high cooling system in order to move out the heat generated by high voltage electric current.PreparationPreparation of sample is quick and simple.Complex preparation.FilamentNo filament used.Tungsten filament is used.Radiation leakageNo radiation risk.There is the risk of radiation leakage.AvailabilityEasily available and cheaper in rate.Not easily available and expensive.VisibilityLiving, as well as the dead sample, can be viewed.Only dead (fixed) organisms can be viewed.Studying the detailed structure of an organism is difficult.3D structure is obtained due to which it is easy to study the structural and other details of organisms.The natural colour of specimen is obtained.Only black and white image is obtained.The image can be seen directly.Image is seen only on fluorescent screen.Definition of Light MicroscopeThe instrument used in labs to observe and study smaller organisms is called a microscope. Light microscope contains an Eyepiece (Ocular lens), tube, coarse focus, fine focus, resolving nose piece, objective, stage clips, diaphragm, mirror, light source, condenser, three or four objective lenses. The light microscope uses the visible light as the source to view the object, along with glass lenses/transparent lenses and projection screen. As these microscopes are easy to handle and simple & easy in working. They can be commonly seen in schools, colleges labs, doctors clinic. The microscope is based on its resolving power, magnification, lenses used, source to view the object. “Resolving power” is the most important, which is the ability to differentiate two very small and closely attached objects clearly. Lesser the distance between the objects, finer will be the result. Light Microscope also referred as Optical Microscope can be classified as Simple and Compound Microscope. In the simple type single lens like magnifying glass are only used, whereas in compound type several lenses are used to magnify the objects clearly. Types of Light (compound) Microscope
Advantages and DisadvantagesFollowing are the advantages and disadvantages of Light Microscope
Disadvantages
Definition of Electron MicroscopeNowadays, an electron microscope is much widely used by scientists and in research labs to get the keen knowledge of even the smallest microorganisms as well as to study all their characteristics in detail. As the name suggests, Electron Microscope uses electrons instead of visible light source to view the objects. Electrons microscopes are the most advanced type of microscopes. In the year 1920, it was recognized that electrons when moved in a vacuum, they behave like “light”. They travel in straight lines and have wavelike properties, with a wavelength much shorter than that of visible light. Types of Electron Microscope
Advantages and DisadvantagesFollowing are the advantages and disadvantages of Electron Microscope
Disadvantages
Key Differences Between Light Microscope and Electron MicroscopeFollowing are the main differences between Light Microscope and Electron Microscope:
ConclusionThough both the microscopes are important and have some positive and negative factors, nowadays Electron Microscopes are broadly used by scientists in a research laboratory to do the detailed study of organisms whereas Light Microscopes are used by schools, colleges, path labs for viewing the organisms which are easily visible through it. Even earlier we were unaware of the diseases like tuberculosis, typhoid, dysentery, measles, etc. as well their causes and remedies, but since the time of the invention of the microscope, scientists were able to resolve them. What are the 3 main differences between light and electron microscopes?Differences between Light Microscope and Electron Microscope. What is difference between electron microscope and light microscope?Electron microscopes differ from light microscopes in that they produce an image of a specimen by using a beam of electrons rather than a beam of light. Electrons have much a shorter wavelength than visible light, and this allows electron microscopes to produce higher-resolution images than standard light microscopes.
What are 2 differences between a light microscope and a scanning electron microscope?Key Differences Between Optical Microscopy vs SEM
Optical microscopes are easy to use, where samples can be analyzed in air or water and the resulting images are in natural color. SEMs are typically larger and operate in a vacuum, which can increase the time to image a sample. Plus, the resulting image is grey-scaled.
What is the main difference between a light and electron microscope multiple choice question?Explanation: The resolving power of the electron microscope is more than 100 times that of the light microscope, and it produces useful magnification up to 400,000X.
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