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Biology-stevens
Terms in this set (3)
Purpose:
In this investigation, you will learn the parts of a compound light microscope, the functions of those parts, and the proper use and care of the microscope. You will also learn the technique of preparing wet-mount slides.
Problem:
What
is the proper use of a compound light microscope?
Pre-Lab Discussion
1. Why might it be a good idea to keep your microscope at least 10 cm from the table?
2. Why should a microscope slide and coverslip be held by their edges?
3. Why do scientists use microscopes?
4. Why should you use lens paper only once?
5. Why is it important to eliminate air bubbles from the slide?
1. To keep from knocking
the microscope off the table.
2. To prevent fingerprints and smudges from getting on them and interfering with the view of the object under the microscope.
3. To study things not easily visible to the naked eye.
4. Lens paper that has been used can get dust that could damage the next lens on which it is used.
5. Air bubbles might cause changes or blur the image.
Analysis
1. Why do you place one hand under the base of the microscope
as you carry it?
2. How is the image of an object seen through the high-power objective different from the image seen through the low-power objective?
3. How does the position of the letter "e" as seen through the microscope differ from the way an "e" normally appears?
4. Explain why a specimen to be viewed under the microscope must be thin.
5. Why should you never use coarse adjustment when focusing on the high-power objective lens?
6. Suppose you were observing an organism
through the microscope and noticed that it moved toward the bottom of the slide and then it moved to the right. What does this tell you about the actual movement of the organism?
1. To make sure the microscope doesn't fall
2. The high-power will have more of a narrow view, but the image will seem larger and more detailed than an image seen through the low-power objective.
3. The letter "e" is upside down and backward.
4.Light must be able to pass
through the specimen in order for you to see detail. The light is shining from the mirror below the stage, through the specimen, the objective lens, the body of the microscope, and the eyepiece to your eye.
5. This could harm and scratch the lens. The high-power objective lens is the longest lens, so using coarse adjustment could smash it into the slide.
6. The microscope makes it appear to move in a direction exactly opposite to that of their actual movement on the slide. The organism
originally actually moved toward the top of the slide and then to the left.
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