A student views some cheek cells under low power before switching to high power, the student should

Microscope Review

The diagram represents a cell in the field of view of a compound light microscope. In which direction should the slide be moved on the microscope stage to center the cell in the field of view? down towards C

A student views some cheek cells under low power. Before switching to high power, the student should
Center the image being
Focus with the coarse adjustment

A student changes the objective of a microscope from 10x to 50x. If this is the only change made, what will happen to the field of view?
The field of view will decrease.
The amount of light will decrease.

When an onion cell is stained with iodine, which organelle becomes more visible under the compound light microscope?

nucleus

To locate a specimen on a prepared slide with a compound microscope, Why must a student should begin with the lowpower objective rather than the highpower objective?
The field of vision is larger under low power than under high po.er

1. Which substance could be added to the slides to make the details more visible? a stain
2. What is the name of the stain used for animal cells? methylene blue
3. What is the name of the stain used for plant cells? iodine The diagram represents the field of view of a compound light microscope. Three unicellular organisms are located across the diameter of the field. What is the approximate length of each unicellular organism?

500um

Give the name and function of each structure labeled.
A – Eyepiece / Ocular: structure you look through
B – Fine adjustment: used to focus under high power
C – Arm – used to hold the microscope D –

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Microscope Review

Give the name and function of each structure labeled. A – Ocular: eyepiece used to look at specimen B – Fine adjustment: to focus specimen under high power C – Arm: used to hold microscope D – Objective lens: used to magnify image E – Coarse adjustment: Used to focus specimen under low power F – Diaphragm – adjust amount of light reaching specimen

E

F

What was the highest possible magnification that can be obtained when using this microscope? Objective x Ocular 40 x 10 = 400x

E

F

What happens to the amount of light in the field of view when switching from low to high power?

E

The amount of light decreases (so the field of view gets darker) F

What happens to the field of view when switching from low to high power?

E

The field of view decreases ( so you see less of the slide). F

Which structure can only be used to focus the specimen under high power? Support your answer. The fine adjustment because using the coarse adjustment under high power can break the objective lens or the slide.

E

F

What are two possible adjustments that need to be made when switching to high power? Adjust the diaphragm to make the field of view brighter. Focus with the fine adjustment.

What happens to the number of cells that are visible in the field of view when switching to high power? The number of cells that are visible in the field of view decreases.

What must be done to the microscope before switching from low power to high power? Center the specimen Focus with the coarse adjustment

The diagram represents a cell in the field of view of a compound light microscope. In which direction should the slide be moved on the microscope stage to center the cell in the field of view?

Towards C

The image to the left is placed on the slide. A student then views the letter “f” under low power. Which diagram below most closely resembles the image under low power?

A student sees the image to the left when observing the letter "f" with the low-power objective lens of a microscope. Which diagram below most closely resembles the image the student will see after switching to high power?

The total magnification of an image is the result of the combined magnifications of the 1) eyepiece and diaphragm 2) objective and eyepiece 3) objective and mirror 4) low-power and high-power objectives

A student views some cheek cells under low power. Before switching to high power, the student should (1) adjust the eyepiece (2) center the image being viewed (3) remove the slide from the stage (4) remove the coverslip

A student changes the objective of a microscope from 10x to 50x. If this is the only change made, what will happen to the field of view? (1) Its diameter will decrease. (2) Its diameter will increase. (3) Its brightness will increase. (4) Its brightness will remain the same.

A student determined that exactly 10 pollen grains could fit along the diameter of the field of view of a microscope. If each pollen grain has a diameter of 200 micrometers, what is the diameter in millimeters of this microscope's field of view? (1) 2,000 mm (2) 2 mm (3) 5 mm (4) 500 mm

To locate a specimen on a prepared slide with a compound microscope, a student should begin with the low-power objective rather than the highpower objective because the (1) field of vision is smaller under low power than under high power (2) field of vision is larger under low power than under high power (3) specimen does not need to be stained for observation under low power but must be stained for observation under high power (4) amount of the specimen that can be observed under low power is less than the amount that can be observed under high power

After switching from the high-power to the low-power objective lens of a compound light microscope, the area of the low-power field will appear (1) larger and brighter (2) smaller and brighter (3) larger and darker (4) smaller and darker

The diagram represents a hydra as viewed with a compound light microscope. If the hydra moves to the right of the slide preparation, which diagram below best represents what will be viewed through the microscope?

Which part of a microscope should be used with the low power objective, but not with the high-power objective? a. fine adjustment b. diaphragm c. coarse adjustment d. eyepiece

A prepared slide was placed on the stage of a microscope so that the slide faced the student, as shown in the diagram below.

Which image will the student most likely see?

Convert: 1. 6.23mm 2. 5678um 3. .093mm 4. 48.2um

6230 = ______ um 5.678 = ______ mm 93 = ______ um .0482 = ______ mm

The diagram represents the field of view of a compound light microscope. Three unicellular organisms are located across the diameter of the field. What is the approximate length of each unicellular organism? 1) 250 um

2) 500 um 3) 1,000 um 4) 1,500 um

This organism could be centered in the field of view by moving the microscope slide towards (1) A (2) B (3) C (4) D

250um

500um

125um

Cell is approximately 375um

1mm 1000um

The diameter is 1mm. What is the length of the cell in um?

1750um

3.5mm 3500um

The length of the cell is approximately 1750um.

There are 3 cells across the field of view. 1500/3 = 500um

Field of view = 1.5mm

= 1500um

When a student uses a compound microscope and switches from low power to high power?

Changing from low power to high power increases the magnification of a specimen. The amount an image is magnified is equal to the magnification of the ocular lens, or eyepiece, multiplied by the magnification of the objective lens. Usually, the ocular lens has a magnification of 10x.

How should a student adjust the microscope in order to view a greater portion of the root tip?

how should a student adjust the microscope in order to view a greater portion of the root tip? switch to a lower power and decrease the size of the opening of the diaphragm.

Which part of a compound light microscope should a student adjust to allow more light?

Look through the eyepiece (1) and move the focus knob until the image comes into focus. Adjust the condenser (7) and light intensity for the greatest amount of light. Move the microscope slide around until the sample is in the centre of the field of view (what you see).

Why can some cells not be seen underneath a microscope?

There is little in the contents of most cells (which are 70% water by weight) to impede the passage of light rays. Thus, most cells in their natural state, even if fixed and sectioned, are almost invisible in an ordinary light microscope. One way to make them visible is to stain them with dyes.

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